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KMID : 0385520220350040181
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Analytical Science & Technology
2022 Volume.35 No. 4 p.181 ~ p.188
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Comparative analysis of yeast cell viability at exponential and stationary growth phases
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An Ye-Jin
Jo Na-Yoon
Kim Hye-Ji
Son Woo-Rim
Park Jin-Kyu
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Abstract
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This paper describes a comparative analysis of yeast cell viability at exponential and stationary growth phases using multiple conventional techniques and statistical tools. Overall, cellular responses to various viability assays were asynchronous. Results of optical density measurement and direct cell counting were asynchronous both at exponential and stationary phases. Proliferative capacity measurement using SP-SDS indicated that cells at the end of the stationary phase were proliferative as much as exponentially growing cells. Metabolic activity assays using two different dyes concluded that the inside of cells at stationary phase is slightly less reducing compared to that of exponentially growing cells, implying that the metabolic activity imperceptibly declined as cells were aged. These results will be helpful to understand the details of yeast cell viability at exponential and stationary growth phases.
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KEYWORD
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yeast, cell viability, exponential phase, stationary phase, proliferative capacity, metabolic activity
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